The Mancias Lab is hiring research technicians! Join our team to work on projects related to pancreatic cancer, autophagy, and iron. Apply via the links below.

The individuals will be an important member of our research team and will be involved in assisting research projects utilizing several techniques that are fundamental to the workflow of the Mancias Lab. In addition to the bench-based research, responsibilities will also include some aspects of laboratory management and organization. Training is available in all aspects of experimental research.

This is a great position for an individual interested in attending a biomedical graduate school program or medical school (or MD/PhD program). Mentoring for graduate/medical school applications is available through the principal investigator as well as clinical shadowing opportunities. Prior research technicians in this position have gone on to medical school and graduate school and have been first author and/or co-author on peer-reviewed publications by the time they left the laboratory.

https://social.icims.com/viewjob/pe164321406814123f60

For a candidate with some experience handling animal models (mouse models):
https://social.icims.com/viewjob/pe164321389274431922

Our lab and the lab of Steven Gygi, Ph.D. at Harvard Medical School are co-hiring a fully funded postdoctoral fellow to begin as early as January 2022. For additional details, please see the post to the left or click on this link:

​https://careers-dfci.icims.com/jobs/26451/research-fellow---mancias-lab/job?mode=view&mobile=false&width=791&height=500&bga=true&needsRedirect=false&jan1offset=-300&jun1offset=-240

Please apply via the link or by e-mail: joseph_mancias@dfci.harvard.edu with a Curriculum Vitae and a cover letter.

​We look forward to hearing from you!

This past week we received our official notice of award for our R01 submission to the NIH/NIDDK! The lab is excited to study 'The role of NCOA4-mediated ferritinophagy in iron homeostasis and ferroptosis.'  From our project summary:

 Specific Aim 1 will define the biochemical mechanisms regulating NCOA4 activity in cells using an integrated quantitative proteomic and cell biologic experimentation workflow. In specific aim 2, we will determine the temporal and spatial dependency of ferritinophagy in vivo for regulating systemic iron homeostasis and erythropoiesis using novel mouse models of NCOA4 knockout and over-expression. Finally, specific aim 3 will focus on examining a role for NCOA4 in regulating sensitivity to ferroptosis in vivo under pathophysiologic conditions, including pancreatic cancer. Together, the proposed in vitro and in vivo studies will identify fundamental roles of NCOA4 in sensing and responding to iron deficiency and overload and determine the utility of pursuing NCOA4 as a therapeutic target for multiple iron-related disorders.

​

Our first bioRxiv pre-print! KRASG12C inhibitors are now in clinical trials for oncogenic KRASG12C-driven tumors including Pancreatic Cancer and Lung Cancer and patients are showing responses. As one concern based on experience with other targeted therapies is development of resistance, we modeled development of adaptation/resistance to KRASG12C pharmacologic inhibition and used mass-spectrometry-based quantitative temporal proteomics to understand how adaptation to these inhibitors could develop over time. We next used this proteomic information to design and test drug combinations to enhance response to KRASG12C monotherapy. Combinations with CDK4/6i and HSP90i showed promise but would require further extensive pre-clinical testing to validate. All of our quantitative temporal proteomic data is available via an interactive website:
​ https://manciaslab.shinyapps.io/KRASi/.

​We’re excited to get feedback via the pre-print process! Great work from co-first authors Naiara Santana-Codina and Amrita Singh Chandhoke! Thanks to collaborators, Drs. Nathanael Gray, Wojciech Fendler and Andy Aguirre. 

Naiara Santana-Codina presents work from the lab at the 2019 AACR Pancreatic Cancer: Advances in Science and Clinical Care meeting on using quantitative temporal proteomics to identify combinatorial treatment strategies in Pancreatic Cancer. Great work Naiara!